Recombinant CD4 anticorps

N° du produit ABIN7072528

Cet anticorps Lapin Chimeric détecte spécifiquement CD4 dans FACS, ELISA et Neut. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant CD4 anticorps (ABIN7072528)

Antigène: CD4

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp CD4 est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Neutralization (Neut)

Clone: DB81

Détail du produit anti-CD4 anticorps

Fonction: Anti-CD4 [DB81], Rabbit IgG, kappa

Specificité: This antibody is specific for human CD4, and recognises an epitope in the vicinity of a 13-amino acid loop in domain 2 of CD4. It cross-reacts with chimpanzee CD4, but not baboon or macaque CD4.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising BALB/c mice with human CD4 complexed with HIV-1 Env (gp120).

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody preferentially binds to a complex-enhanced epitope on the D2 domain of human CD4. Plasmon resonance analysis has shown that the binding affinity of this antibody to the CD4-gp120 complex is almost one order of magnitude higher than its binding to CD4 alone (Burastero et al, 2011), while ELISA analysis has confirmed that the epitope recognised by this antibody does not involve gp120 (Burastero et al, 2011). This antibody, through a post-binding mechanism, inhibits the cell fusion and replication of a range of HIV-1 strains (Burastero et al, 2011). It does not exert inhibitory effects on T-cell activation in vitro, a useful property for clinical use.

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CD4

Antigène: CD4

Autre désignation: CD4

Sujet: Lu/T4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events